2023-04-28 14:28:49 INFO: ***** Start a BUSCO v5.4.7 analysis, current time: 04/28/2023 14:28:49 ***** 2023-04-28 14:28:49 INFO: Configuring BUSCO with local environment 2023-04-28 14:28:49 WARNING: Running Auto Lineage Selector as no lineage dataset was specified. This will take a little longer than normal. If you know what lineage dataset you want to use, please specify this in the config file or using the -l (--lineage-dataset) flag in the command line. 2023-04-28 14:28:49 INFO: Mode is genome 2023-04-28 14:28:49 INFO: 'Force' option selected; overwriting previous results directory 2023-04-28 14:28:50 INFO: Downloading information on latest versions of BUSCO data... 2023-04-28 14:28:52 INFO: Input file is /busco_wd/test_data/eukaryota/genome.fna 2023-04-28 14:28:52 INFO: No lineage specified. Running lineage auto selector. 2023-04-28 14:28:52 INFO: ***** Starting Auto Select Lineage ***** This process runs BUSCO on the generic lineage datasets for the domains archaea, bacteria and eukaryota. Once the optimal domain is selected, BUSCO automatically attempts to find the most appropriate BUSCO dataset to use based on phylogenetic placement. --auto-lineage-euk and --auto-lineage-prok are also available if you know your input assembly is, or is not, an eukaryote. See the user guide for more information. A reminder: Busco evaluations are valid when an appropriate dataset is used, i.e., the dataset belongs to the lineage of the species to test. Because of overlapping markers/spurious matches among domains, busco matches in another domain do not necessarily mean that your genome/proteome contains sequences from this domain. However, a high busco score in multiple domains might help you identify possible contaminations. 2023-04-28 14:28:52 INFO: Running BUSCO using lineage dataset archaea_odb10 (prokaryota, 2021-02-23) 2023-04-28 14:28:52 INFO: Running 1 job(s) on bbtools, starting at 04/28/2023 14:28:52 2023-04-28 14:28:53 INFO: [bbtools] 1 of 1 task(s) completed 2023-04-28 14:28:53 INFO: ***** Run Prodigal on input to predict and extract genes ***** 2023-04-28 14:28:53 INFO: Running Prodigal with genetic code 11 in single mode 2023-04-28 14:28:53 INFO: Running 1 job(s) on prodigal, starting at 04/28/2023 14:28:53 2023-04-28 14:28:54 INFO: [prodigal] 1 of 1 task(s) completed 2023-04-28 14:28:54 INFO: Genetic code 11 selected as optimal 2023-04-28 14:28:54 INFO: ***** Run HMMER on gene sequences ***** 2023-04-28 14:28:54 INFO: Running 194 job(s) on hmmsearch, starting at 04/28/2023 14:28:54 2023-04-28 14:28:56 INFO: [hmmsearch] 20 of 194 task(s) completed 2023-04-28 14:28:56 INFO: [hmmsearch] 39 of 194 task(s) completed 2023-04-28 14:28:56 INFO: [hmmsearch] 59 of 194 task(s) completed 2023-04-28 14:28:57 INFO: [hmmsearch] 78 of 194 task(s) completed 2023-04-28 14:28:57 INFO: [hmmsearch] 97 of 194 task(s) completed 2023-04-28 14:28:57 INFO: [hmmsearch] 117 of 194 task(s) completed 2023-04-28 14:28:57 INFO: [hmmsearch] 136 of 194 task(s) completed 2023-04-28 14:28:57 INFO: [hmmsearch] 156 of 194 task(s) completed 2023-04-28 14:28:57 INFO: [hmmsearch] 175 of 194 task(s) completed 2023-04-28 14:28:57 INFO: [hmmsearch] 194 of 194 task(s) completed 2023-04-28 14:29:00 INFO: Results: C:1.0%[S:1.0%,D:0.0%],F:0.5%,M:98.5%,n:194 2023-04-28 14:29:00 INFO: Running BUSCO using lineage dataset bacteria_odb10 (prokaryota, 2020-03-06) 2023-04-28 14:29:00 INFO: Running 1 job(s) on bbtools, starting at 04/28/2023 14:29:00 2023-04-28 14:29:02 INFO: [bbtools] 1 of 1 task(s) completed 2023-04-28 14:29:02 INFO: ***** Run Prodigal on input to predict and extract genes ***** 2023-04-28 14:29:02 INFO: Genetic code 11 selected as optimal 2023-04-28 14:29:02 INFO: ***** Run HMMER on gene sequences ***** 2023-04-28 14:29:02 INFO: Running 124 job(s) on hmmsearch, starting at 04/28/2023 14:29:02 2023-04-28 14:29:03 INFO: [hmmsearch] 13 of 124 task(s) completed 2023-04-28 14:29:03 INFO: [hmmsearch] 25 of 124 task(s) completed 2023-04-28 14:29:03 INFO: [hmmsearch] 50 of 124 task(s) completed 2023-04-28 14:29:03 INFO: [hmmsearch] 63 of 124 task(s) completed 2023-04-28 14:29:03 INFO: [hmmsearch] 75 of 124 task(s) completed 2023-04-28 14:29:03 INFO: [hmmsearch] 87 of 124 task(s) completed 2023-04-28 14:29:03 INFO: [hmmsearch] 100 of 124 task(s) completed 2023-04-28 14:29:03 INFO: [hmmsearch] 112 of 124 task(s) completed 2023-04-28 14:29:03 INFO: [hmmsearch] 124 of 124 task(s) completed 2023-04-28 14:29:05 WARNING: BUSCO did not find any match. Make sure to check the log files if this is unexpected. 2023-04-28 14:29:05 INFO: Results: C:0.0%[S:0.0%,D:0.0%],F:0.0%,M:100.0%,n:124 2023-04-28 14:29:05 INFO: Running BUSCO using lineage dataset eukaryota_odb10 (eukaryota, 2020-09-10) 2023-04-28 14:29:05 INFO: Running 1 job(s) on bbtools, starting at 04/28/2023 14:29:05 2023-04-28 14:29:07 INFO: [bbtools] 1 of 1 task(s) completed 2023-04-28 14:29:07 INFO: Running 1 job(s) on metaeuk, starting at 04/28/2023 14:29:07 2023-04-28 14:29:49 INFO: [metaeuk] 1 of 1 task(s) completed 2023-04-28 14:29:51 INFO: ***** Run HMMER on gene sequences ***** 2023-04-28 14:29:51 INFO: Running 255 job(s) on hmmsearch, starting at 04/28/2023 14:29:51 2023-04-28 14:29:52 INFO: [hmmsearch] 51 of 255 task(s) completed 2023-04-28 14:29:53 INFO: [hmmsearch] 77 of 255 task(s) completed 2023-04-28 14:29:53 INFO: [hmmsearch] 102 of 255 task(s) completed 2023-04-28 14:29:53 INFO: [hmmsearch] 128 of 255 task(s) completed 2023-04-28 14:29:53 INFO: [hmmsearch] 153 of 255 task(s) completed 2023-04-28 14:29:53 INFO: [hmmsearch] 179 of 255 task(s) completed 2023-04-28 14:29:53 INFO: [hmmsearch] 204 of 255 task(s) completed 2023-04-28 14:29:54 INFO: [hmmsearch] 230 of 255 task(s) completed 2023-04-28 14:29:54 INFO: [hmmsearch] 255 of 255 task(s) completed 2023-04-28 14:29:56 INFO: Validating exons and removing overlapping matches 2023-04-28 14:29:57 INFO: 0 candidate overlapping regions found 2023-04-28 14:29:57 INFO: 51 exons in total 2023-04-28 14:29:57 INFO: Results: C:19.2%[S:19.2%,D:0.0%],F:0.8%,M:80.0%,n:255 2023-04-28 14:29:57 INFO: Extracting missing and fragmented buscos from the file refseq_db.faa... 2023-04-28 14:30:14 INFO: Running 1 job(s) on metaeuk, starting at 04/28/2023 14:30:14 2023-04-28 14:30:45 INFO: [metaeuk] 1 of 1 task(s) completed 2023-04-28 14:30:48 INFO: ***** Run HMMER on gene sequences ***** 2023-04-28 14:30:48 INFO: Running 206 job(s) on hmmsearch, starting at 04/28/2023 14:30:48 2023-04-28 14:30:50 INFO: [hmmsearch] 21 of 206 task(s) completed 2023-04-28 14:30:50 INFO: [hmmsearch] 42 of 206 task(s) completed 2023-04-28 14:30:50 INFO: [hmmsearch] 62 of 206 task(s) completed 2023-04-28 14:30:51 INFO: [hmmsearch] 83 of 206 task(s) completed 2023-04-28 14:30:51 INFO: [hmmsearch] 104 of 206 task(s) completed 2023-04-28 14:30:51 INFO: [hmmsearch] 124 of 206 task(s) completed 2023-04-28 14:30:51 INFO: [hmmsearch] 145 of 206 task(s) completed 2023-04-28 14:30:51 INFO: [hmmsearch] 165 of 206 task(s) completed 2023-04-28 14:30:51 INFO: [hmmsearch] 186 of 206 task(s) completed 2023-04-28 14:30:52 INFO: [hmmsearch] 206 of 206 task(s) completed 2023-04-28 14:30:54 INFO: Validating exons and removing overlapping matches 2023-04-28 14:30:56 INFO: 0 candidate overlapping regions found 2023-04-28 14:30:56 INFO: 51 exons in total 2023-04-28 14:30:56 INFO: Results: C:19.2%[S:19.2%,D:0.0%],F:0.8%,M:80.0%,n:255 2023-04-28 14:30:56 INFO: eukaryota_odb10 selected 2023-04-28 14:30:56 INFO: ***** Searching tree for chosen lineage to find best taxonomic match ***** 2023-04-28 14:30:56 INFO: Extract markers... 2023-04-28 14:30:56 INFO: Place the markers on the reference tree... 2023-04-28 14:30:56 INFO: Running 1 job(s) on sepp, starting at 04/28/2023 14:30:56 2023-04-28 14:35:03 INFO: [sepp] 1 of 1 task(s) completed 2023-04-28 14:35:03 INFO: Lineage saccharomycetes is selected, supported by 18 markers out of 19 2023-04-28 14:35:04 INFO: Running BUSCO using lineage dataset saccharomycetes_odb10 (eukaryota, 2020-08-05) 2023-04-28 14:35:04 INFO: Running 1 job(s) on bbtools, starting at 04/28/2023 14:35:04 2023-04-28 14:35:06 INFO: [bbtools] 1 of 1 task(s) completed 2023-04-28 14:35:06 INFO: Running 1 job(s) on metaeuk, starting at 04/28/2023 14:35:06 2023-04-28 14:35:13 INFO: [metaeuk] 1 of 1 task(s) completed 2023-04-28 14:35:15 INFO: ***** Run HMMER on gene sequences ***** 2023-04-28 14:35:15 INFO: Running 2137 job(s) on hmmsearch, starting at 04/28/2023 14:35:15 2023-04-28 14:35:18 INFO: [hmmsearch] 214 of 2137 task(s) completed 2023-04-28 14:35:20 INFO: [hmmsearch] 428 of 2137 task(s) completed 2023-04-28 14:35:22 INFO: [hmmsearch] 642 of 2137 task(s) completed 2023-04-28 14:35:24 INFO: [hmmsearch] 855 of 2137 task(s) completed 2023-04-28 14:35:26 INFO: [hmmsearch] 1069 of 2137 task(s) completed 2023-04-28 14:35:28 INFO: [hmmsearch] 1283 of 2137 task(s) completed 2023-04-28 14:35:30 INFO: [hmmsearch] 1496 of 2137 task(s) completed 2023-04-28 14:35:32 INFO: [hmmsearch] 1710 of 2137 task(s) completed 2023-04-28 14:35:34 INFO: [hmmsearch] 1924 of 2137 task(s) completed 2023-04-28 14:35:38 INFO: [hmmsearch] 2137 of 2137 task(s) completed 2023-04-28 14:35:43 INFO: Validating exons and removing overlapping matches 2023-04-28 14:35:45 INFO: 0 candidate overlapping regions found 2023-04-28 14:35:45 INFO: 45 exons in total 2023-04-28 14:35:45 INFO: Extracting missing and fragmented buscos from the file refseq_db.faa... 2023-04-28 14:35:50 INFO: Running 1 job(s) on metaeuk, starting at 04/28/2023 14:35:50 2023-04-28 14:35:57 INFO: [metaeuk] 1 of 1 task(s) completed 2023-04-28 14:35:59 INFO: ***** Run HMMER on gene sequences ***** 2023-04-28 14:35:59 INFO: Running 2093 job(s) on hmmsearch, starting at 04/28/2023 14:35:59 2023-04-28 14:36:03 INFO: [hmmsearch] 210 of 2093 task(s) completed 2023-04-28 14:36:05 INFO: [hmmsearch] 419 of 2093 task(s) completed 2023-04-28 14:36:06 INFO: [hmmsearch] 628 of 2093 task(s) completed 2023-04-28 14:36:08 INFO: [hmmsearch] 838 of 2093 task(s) completed 2023-04-28 14:36:09 INFO: [hmmsearch] 1047 of 2093 task(s) completed 2023-04-28 14:36:10 INFO: [hmmsearch] 1256 of 2093 task(s) completed 2023-04-28 14:36:11 INFO: [hmmsearch] 1466 of 2093 task(s) completed 2023-04-28 14:36:12 INFO: [hmmsearch] 1675 of 2093 task(s) completed 2023-04-28 14:36:13 INFO: [hmmsearch] 1884 of 2093 task(s) completed 2023-04-28 14:36:14 INFO: [hmmsearch] 2093 of 2093 task(s) completed 2023-04-28 14:36:16 INFO: Validating exons and removing overlapping matches 2023-04-28 14:36:18 INFO: 3 candidate overlapping regions found 2023-04-28 14:36:18 INFO: 49 exons in total 2023-04-28 14:36:20 INFO: Results: C:2.1%[S:2.1%,D:0.0%],F:0.0%,M:97.9%,n:2137 2023-04-28 14:36:20 INFO: -------------------------------------------------- |Results from generic domain eukaryota_odb10 | -------------------------------------------------- |C:19.2%[S:19.2%,D:0.0%],F:0.8%,M:80.0%,n:255 | |49 Complete BUSCOs (C) | |49 Complete and single-copy BUSCOs (S) | |0 Complete and duplicated BUSCOs (D) | |2 Fragmented BUSCOs (F) | |204 Missing BUSCOs (M) | |255 Total BUSCO groups searched | -------------------------------------------------- -------------------------------------------------- |Results from dataset saccharomycetes_odb10 | -------------------------------------------------- |C:2.1%[S:2.1%,D:0.0%],F:0.0%,M:97.9%,n:2137 | |45 Complete BUSCOs (C) | |45 Complete and single-copy BUSCOs (S) | |0 Complete and duplicated BUSCOs (D) | |1 Fragmented BUSCOs (F) | |2091 Missing BUSCOs (M) | |2137 Total BUSCO groups searched | -------------------------------------------------- 2023-04-28 14:36:20 INFO: BUSCO analysis done with WARNING(s). Total running time: 449 seconds ***** Summary of warnings: ***** 2023-04-28 14:28:49 WARNING:busco.BuscoConfig Running Auto Lineage Selector as no lineage dataset was specified. This will take a little longer than normal. If you know what lineage dataset you want to use, please specify this in the config file or using the -l (--lineage-dataset) flag in the command line. 2023-04-28 14:29:05 WARNING:busco.busco_tools.hmmer BUSCO did not find any match. Make sure to check the log files if this is unexpected. 2023-04-28 14:36:20 INFO: Results written in /busco_wd/test_eukaryota 2023-04-28 14:36:20 INFO: For assistance with interpreting the results, please consult the userguide: https://busco.ezlab.org/busco_userguide.html 2023-04-28 14:36:20 INFO: Visit this page https://gitlab.com/ezlab/busco#how-to-cite-busco to see how to cite BUSCO